📊 EMBOSS Pepinfo: Basic Protein Sequence Information

EMBOSS Pepinfo is a bioinformatics tool that provides basic information and statistics about a protein sequence. It's a quick utility for obtaining fundamental properties such as molecular weight, amino acid counts, and the overall charge of a protein.

❓ What is EMBOSS Pepinfo?

Pepinfo analyzes an amino acid sequence and reports its length, molecular weight, isoelectric point (pI), and amino acid composition. It's a simpler tool compared to Pepstats or SAPS, designed for a rapid overview of a protein's fundamental characteristics.

  • Basic Statistics: Calculates molecular weight, pI, and amino acid counts.

  • Quick Overview: Provides essential information for protein characterization.

  • Simple & Fast: Designed for rapid assessment of protein properties.

🎯 Why Use Pepinfo? For Quick Protein Property Checks

EMBOSS Pepinfo is indispensable for:

  • 🔍 Initial Protein Characterization: Getting a fast summary of a protein's basic physicochemical properties.

  • 🧬 Molecular Weight Verification: Confirming the molecular weight of a protein for gel electrophoresis or mass spectrometry.

  • 📊 Amino Acid Composition Analysis: Quickly checking the composition of a protein for specific residue biases.

  • 📈 Educational Purposes: Demonstrating fundamental protein properties.

  • 🎯 Simple Data Preparation: Obtaining basic information needed for downstream tools or reports.

🧑‍💻 How to Use EMBOSS Pepinfo on Job Dispatcher: A Step-by-Step Guide

Follow these simple steps to get basic information about your protein sequence(s) with Pepinfo:

1️⃣ Navigate to the Tool

  1. From the main menu, go to All Tools (or search for "EMBOSS Pepinfo").

  2. Click the prominent Use Tool button located next to "EMBOSS Pepinfo."

2️⃣ Input Your Protein Sequence

  • Locate the input box (large text area) or the "upload a Sequence File" option.

  • Paste your protein sequence(s) in FASTA format or upload a FASTA file.

    >ProteinA_sample
MKVLWAALLVTFLAGCQAKVEQAV

  • Important: You can provide a sequence either by typing into the text area OR by uploading a file, but not both simultaneously. Please clear one input to proceed.

3️⃣ Configure Parameters

  • 📝 Title: Provide a descriptive title for your job (e.g., "My Protein Info Report").

  • 💡 Sequence Type: (Automatically set to Protein for Pepinfo, as it expects protein input).

  • 📏 HYDROPATY WINDOW SIZE (windowsize): Input your own value for the size of the sliding window used for hydropathy calculations (though the primary output is basic stats, this parameter might influence internal calculations or a hidden plot).

    • Default: 7
    • Input type: Number

4️⃣ Submit Your Job

  • Once your sequence is entered and parameters are set, click the Submit or Run button.

  • Your job will be dispatched to the EMBL-EBI Web Service. You will be automatically redirected to a Job Status page to monitor its progress.

5️⃣ Interpret Results

  • On the results page, you will find a concise textual report summarizing the basic properties of your protein sequence(s).
  • The report typically includes sequence length, molecular weight, isoelectric point (pI), and a breakdown of amino acid counts.
  • ⭐ Tip: Use Pepinfo for quick checks when you only need fundamental information about your protein, rather than a detailed statistical analysis.

💬 Need Help?

If you run into issues, please visit our Contact Us page for dedicated support. Happy analyzing!